![]() ![]() Antioxidant nanomaterials in advanced diagnoses and treatments of ischemia reperfusion injuries. J., Latifi, S., Pazoki-Toroudi, H., & Akhavan, O. Heart Disease and Stroke Statistics-2020 Update: A report from the American Heart Association. We found that liraglutide may attenuate MI/RI by inhibiting necroptosis, in part by enhancing the activity of the GLP-1R/PI3K/Akt pathway. Both the GLP-1R inhibitor exendin (9–39) and the PI3K inhibitor LY294002 abrogated the protective effects of liraglutide in vitro. Mechanistically, we revealed that liraglutide exerted cardioprotective effects through a glucagon-like peptide-1 receptor (GLP-1R) and phosphatidylinositol-3 kinase (PI3K)-dependent pathway. The levels of necroptosis-associated proteins (receptor-interacting protein kinase 3 (RIPK3), p-RIPK3, and phosphorylated-mixed lineage kinase domain-like protein (p-MLKL)) were also reduced by the liraglutide treatment. The myocardial infarct size and cardiac enzyme release were reduced in the heart tissues from the liraglutide-treated group. Our data revealed that liraglutide resulted in markedly increased cell viability and reduced cardiac enzyme release by protecting cardiomyocytes from a necrosis-like phenotype after H/R. H/R or I/R damage was assessed by performing biochemical assay, Hoechst 33342/PI staining, H&E (hematoxylin and eosin) staining, and Annexin-V/PI staining. ![]() Hypoxia/reoxygenation (H/R) was performed on H9c2 cells in vitro to simulate ischemia/reperfusion (I/R) injury, and an MI/RI rat model was established in vivo by ligating the anterior descending branch of the left coronary artery. This study aimed to explore the effect of liraglutide on MI/RI-induced necroptosis and its potential mechanisms. Researchers do not know whether the cardioprotective effect of liraglutide is involved in regulating necroptosis. Liraglutide is an effective option for the treatment of type 2 diabetes and has recently been reported to exert cardioprotective effects on MI/RI. Necroptosis is a crucial programmed cell death that is tightly associated with myocardial ischemia/reperfusion injury (MI/RI). ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |